Serological comparison of five arginine-utilizing Mycoplasma species by two-dimensional immunoelectrophoresis.

Characterization of the strain-specific and common surface antigens of Mycoplasma arginini.

A combination of quantitative immunoelectrophoresis and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis was used to determine location and molecular weights of surface membrane antigens of four strains of Mycoplasma arginini. Two major surface antigens were identified for M. arginini by absorption of antiserum with whole cells: one surface antigen was strain specific, electropho...

Mycoplasma agalactiae 111-The Comparison of Different Serological Tests with M. agalactiae Antigen

Serological cross-reaction between lipids of Mycoplasma pneumoniae and Mycoplasma neurolyticum.

The complement-fixing activity of crude lipid extracts of 10 Mycoplasma species was compared with that of whole organism antigens employing immune rabbit serum. Five species (M. pneumoniae, M. neurolyticum, M. granularum, M. laidlawii, and M. fermentans) showed serological activity, whereas the remaining five species (M. canis, M. felis, M. gallisepticum, M. hyorhinis, and M. pulmonis) did not ...

Differences in incorporation of nucleic acid bases and nucleosides by various Mycoplasma and Acholeplasma species.

Eight species representative of the serological diversity of the Mycoplasmatales were tested for their ability to incorporate radiolabeled nucleic acid precursors into acid-insoluble material. Cultures in complex growth medium were centrifuged and resuspended in minimal essential medium (Eagle). For Acholeplasma laidlawii, labeling occurred mainly during the first 4 h of incubation, with substr...

Characterization of membrane and cytoplasmic antigens of Mycoplasma arginini by two-dimensional (crossed) immunoelectrophoresis.

Two-dimensional immunoelectrophoresis was employed to electrophoretically identify membrane and cytoplasmic antigens of Mycoplasma arginini G-230. Five distinct cytoplasmic antigens were observed in soluble fractions prepared by digitonin lysis with electrophoretic mobilities (relative to bovine albumin) ranging from 0.36 to 0.86; four of these were common to other M. arginini strains: leonis a...